RESEARCH
Dr. Lee and his group members have been developing the microarray 3D bioprinting technology in predicting human toxicology. Our research is focused on the following areas:
1) Develop 3D human cell printing on microarray chips for tissue engineering and disease modeling
- Creating brain tissue replicas using stem cells for developmental toxicology
- Creating liver tissue replicas via layered hepatic cell printing and human liver organoid culture
- Generating tumor micro-tissues with patient cancer cells for personalized cancer therapy
2) Establish high-content imaging (HCI) with 3D-cultured human cells
3) Synthesize tunable hydrogels (“bioink”) for microarray 3D bioprinting
4) Develop viral gene delivery systems to study individual-dependent, adverse drug responses
Microarray Chip Platform
3D Cell/Tissue Culture on the 384PillarPlate
Advantages of Microarray 3D Bioprinting
- Rapid creation of human tissue constructs via novel microarray 3D printing technology
- Approximately 1000-fold miniaturization of human tissues, saving expensive resources including human cells, growth factors, and extracellular matrices
- High-throughput, systematic study of cellular microenvironments for tissue engineering and regenerative medicines
- High-throughput, high-content imaging of miniaturized human tissue constructs for mechanistic studies
Commercial Applications of Miniaturized Human Tissue Constructs
- Predictive assessment of drug toxicity, efficacy, and pharmacokinetics for preclinical evaluations on bioprinted tissue constructs
- Human disease modeling on bioprinted tissue constructs
- Systematic study of cellular microenvironments for tissue engineering and regenerative medicines
- High-throughput, high-content screening of drug candidates and environmental toxicants
Applications of Microarray Biochips
